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KMID : 0378019740170040515
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New Medical Journal
1974 Volume.17 No. 4 p.515 ~ p.523
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Multiplication of Staph. albus, Staph. aureus, B. subtilis, Es. coli and Ps. aeruginosa in the male and female urine
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Abstract
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It is well known the female cases are about ten times more often than male cases of urinary tract infections and among the causative agents Es. coli is the most frequently isolated species. One of the microbial determinants in establishing infections is the rapidity of adaptation to host environments and multiplication in the host tissues.
The author conceived that the mode of microbial growth and multiplication in the urine might be different depending on the species or strains of the organisms and the host sexes.
Thus, bacterial suspensions prepared from the Brain Heart infusion Broth cultures of Staph. albus, Staph. aureus, B. subtilis, Es. coli and Ps. aeruginosa were inoculated into the normal male and female urines, which were adjusted to pH 6.0 or 7.0 with M/15 solution of KH2PO4 or Na2HPO4,. 12 H2O prior to bacterial inoculation.
Number of viable units in the urine were enumerated after incubation of 1. 3, 5, 7. 10, 15. 20 and 24 hours at 33°C. or 37° C. aerobically.
Results of investigation were as follows:
1) Viable units of Staph. albus began to increase after 3 hours of lag phase and reached the peak number (107-108/ml.) after 15 to 20 hours of incubation.
2) Viable units of Staph. aureus began to increase after the lag phase of 3 hours in the male urine and 1 hour in the female urine and reached the peak number (107-108/ml.) after about 10 hours of incubation. The difference of urinary pH appeared to have more significant effects on the mode of multiplication of Staph. aureus than the difference of temperature.
3) Viable units of B. subtilis began to increase after the lag phase of 1 to 3 hours nd reached the peak number (106-107/ml.) after the incubation for ca. 10 hours.
4) Viable units of Es. coli increased rapidly with noticiable lag phase and reached the peak number (108-109/ml.) after 10 hours in male urine and 7 hours in the female urine.
The difference of incubation temperature appeared to have more significant effects on the mode of multiplication of Es. coli than the difference of urinary pH.
5) Viable units of Ps. aeruginosa began to increase after the lag phase of 3 hours in the male urine and 1 hour in the female urine and continued rather slow increase upto 24 hours of incubation. As the case of Es. coli the difference of incubation temperature appeared to have more significant effects on the mode of multiplication than the urinary pH.
6) Among the species investigated, Es. coli showed the most rapid increase and the highest peak number of urine.
7) All the three species frequently encountered in the urinary tract infection cases, i. e. Es. coli, Staph. aureus, and Ps. aeruginosa, increased more rapidly in the female urine than in the male urine.
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